Nitrosamines and Aromatic Amines
نویسنده
چکیده
A new method for the simultaneous determination of hemoglobin adducts from aromatic amines and tobacco-specific nitrosamines (TSNA) is described. After mild base-catalysed hydrolysis releasing aromatic amines and the TSNA adduct 4-hydroxy-1-(3-pyridyl)-1butanone (HPB), the extraction, cleanup, and concentration are performed by a one-step procedure using C 1 8 cartridges. Determination in the picograms per gram of hemoglobin range by gas chromatography-mass spectrometry with negative chemical ionization requires a separate derivatization procedure with pentafluoropropionic anhydride and pentafluorobenzoylchloride for aromatic amines and HPB, respectively. The method is shown to be quantitative, reproducible, and applicable to the determination of hemoglobin adducts from monocyclic and bicyclic aromatic amines as well as TSNA in smokers and nonsmokers.
منابع مشابه
Copper(II)-catalyzed oxidative N-nitrosation of secondary and tertiary amines with nitromethane under an oxygen atmosphere.
The combination of a catalytic amount of Cu(OTf)2 and less than a stoichiometric amount of 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) under an O2 atmosphere effectively promoted the N-nitrosation of both secondary aromatic/aliphatic amines and tertiary aromatic amines with nitromethane (CH3NO2) leading to the preparation of N-nitrosamine derivatives.
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